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bcl 2 d17c4 rabbit mab  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc bcl 2 d17c4 rabbit mab
    Bcl 2 D17c4 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 2009 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bcl-2+(d17c4)/Bcl-2+Rabbit+mAb/pm41857115-129-14-55
    Average 97 stars, based on 2009 article reviews
    bcl 2 d17c4 rabbit mab - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Exploring the therapeutic mechanism of kaempferol in acute lung injury: Network pharmacology and experimental validation.
    Article Snippet: Acute lung injury (ALI) is a common and life-threatening respiratory disorder characterised by severe inflammation and persistently high mortality rates.. Kaempferol (KAE), a natural flavonoid compound, has diverse biological activities and holds potential for therapeutic applications.. In this study, we employed a network pharmacology approach to predict the mechanism through which KAE mitigates ALI; this mechanism was subsequently validated experimentally in a mouse model of ALI.

    Membrane:

    Article Title: Exploring the therapeutic mechanism of kaempferol in acute lung injury: Network pharmacology and experimental validation.
    Article Snippet: Acute lung injury (ALI) is a common and life-threatening respiratory disorder characterised by severe inflammation and persistently high mortality rates.. Kaempferol (KAE), a natural flavonoid compound, has diverse biological activities and holds potential for therapeutic applications.. In this study, we employed a network pharmacology approach to predict the mechanism through which KAE mitigates ALI; this mechanism was subsequently validated experimentally in a mouse model of ALI.

    Western Blot:

    Article Title: Carnosic Acid Activates the STING/IRF3 Pathway to Induce Nitric Oxide-Mediated Apoptosis in Osteosarcoma Cells.
    Article Snippet: Transient transfections were performed using LipofectamineTM 3000 reagent (Thermo Fisher Scientific, Waltham, MA, USA; Cat# L3000008) according to the manufacturer’s instructions. .. For Western blotting and immunohistochemistry, primary antibodies included antiSTING (CST, Danvers, MA, USA; Cat# 13647), anti-TBK1 (CST, Cat# 3504), anti-p-TBK1 (Ser172, CST, Cat# 5483), anti-IRF-3 (CST, Cat# 11904), anti-p-IRF-3 (Ser396, CST, Cat# 4947), anti-cleaved caspase-3 (CST, Cat# 9664), anti-PARP (CST, Cat# 9542), anti-Bax (CST, Cat# 5023), anti-Bcl-2 (CST, Cat# 3498), anti-Bak (CST, Cat# 12105), anti-Bcl-xL (CST, Cat# 2764), https://doi.org/10.3390/antiox15030374 and anti-α-Tubulin (CST, Cat# 2148). .. Alexa Fluor 488- or 594-conjugated secondary antibodies (Thermo Fisher Scientific, Waltham, MA, USA) were used for immunofluorescence.

    Immunohistochemistry:

    Article Title: Carnosic Acid Activates the STING/IRF3 Pathway to Induce Nitric Oxide-Mediated Apoptosis in Osteosarcoma Cells.
    Article Snippet: Transient transfections were performed using LipofectamineTM 3000 reagent (Thermo Fisher Scientific, Waltham, MA, USA; Cat# L3000008) according to the manufacturer’s instructions. .. For Western blotting and immunohistochemistry, primary antibodies included antiSTING (CST, Danvers, MA, USA; Cat# 13647), anti-TBK1 (CST, Cat# 3504), anti-p-TBK1 (Ser172, CST, Cat# 5483), anti-IRF-3 (CST, Cat# 11904), anti-p-IRF-3 (Ser396, CST, Cat# 4947), anti-cleaved caspase-3 (CST, Cat# 9664), anti-PARP (CST, Cat# 9542), anti-Bax (CST, Cat# 5023), anti-Bcl-2 (CST, Cat# 3498), anti-Bak (CST, Cat# 12105), anti-Bcl-xL (CST, Cat# 2764), https://doi.org/10.3390/antiox15030374 and anti-α-Tubulin (CST, Cat# 2148). .. Alexa Fluor 488- or 594-conjugated secondary antibodies (Thermo Fisher Scientific, Waltham, MA, USA) were used for immunofluorescence.

    Recombinant:

    Article Title: Capicua mediates interleukin-2 withdrawal–induced death of activated CD8+ T cells
    Article Snippet: .. Materials and methods 336 4.1 Reagents and antibodies 337 Reagents and antibodies used in this study were obtained from the indicated sources: 338 Recombinant human IL-2 (SL Pharm, S19991010); Annexin V-FITC/PI Apoptosis Kit 339 (Elabscience, E-CK-A211); Actinomycin D (MCE, HY-17559 ); TrueCut Cas9 340 Protein (Invitrogen, A36498); Nuclear and Cytoplasmic Extraction Reagents 341 (Thermo, 78835); Zombie VioletTM Fixable Viability Kit (BioLegend, 423113); 342 Pacific Blue anti-mouse CD3 Antibody (BioLegend, 100214); FITC anti-mouse CD8a 343 Antibody (BioLegend, 100706); APC anti-mouse CD8a Antibody (BD Pharmingen, 344 553035); PE/Cyanine7 anti-mouse CD3 Antibody (BioLegend, 100220); APC anti-345 mouse IFN-γ Antibody (Invivogen, 17-7311-82); PE-Labeled Mouse H-346 2Kb&B2M&OVA (SIINFEKL) Tetramer Protein (Acrobiosystems, H2A-MP2H7); 347 Capicua Antibody (Novusbio, NB110-59906); anti-FLAG Antibody (Sigma-Aldrich, 348 F3165); β-actin (Sigma-Aldrich, A2228); Bim Antibody (Cell Signaling Technology, 349 2933T); Bcl-2 Antibody (Cell Signaling Technology, 3498T); Bax Antibody (Cell 350 Signaling Technology, 2772T); Bcl2 antibody (Proteintech, 68103-1-Ig); BAX 351 antibody (Proteintech, 60267-1-Ig); KEAP1 antibody (Proteintech, 10503-2-AP); 352 Anti-mouse Ig HRP (Rockland Immunochemicals, 18-8817-33); Anti-rabbit IgG HRP 353 (Rockland Immunochemicals, 18-8816-33); HRP-conjugated Mouse anti-Rabbit IgG 354 Light Chain (ABclonal, AS061); Prestained Protein Ladder 10-180 kDa (Thermo, 355 26616); Prestained Protein Ladder 25-400 kDa (Sevenbio, SW179). .. 356 4.2 Cells 357 CTLL-2 cells were purchased from the Cell Resource Center, Peking Union Medical 358 College (IBMS, CAMS/PUMC) and cultured in RPMI-1640 (Gibco) supplemented 359 with 10% fetal bovine serum (FBS) and 1× penicillin–streptomycin (Invitrogen), 10 360 mM HEPES, 1 mM Sodium pyruvate (Gibco), 2mM GlutaMAX (Gibco), and 100 361 U/ml IL-2.

    Extraction:

    Article Title: Capicua mediates interleukin-2 withdrawal–induced death of activated CD8+ T cells
    Article Snippet: .. Materials and methods 336 4.1 Reagents and antibodies 337 Reagents and antibodies used in this study were obtained from the indicated sources: 338 Recombinant human IL-2 (SL Pharm, S19991010); Annexin V-FITC/PI Apoptosis Kit 339 (Elabscience, E-CK-A211); Actinomycin D (MCE, HY-17559 ); TrueCut Cas9 340 Protein (Invitrogen, A36498); Nuclear and Cytoplasmic Extraction Reagents 341 (Thermo, 78835); Zombie VioletTM Fixable Viability Kit (BioLegend, 423113); 342 Pacific Blue anti-mouse CD3 Antibody (BioLegend, 100214); FITC anti-mouse CD8a 343 Antibody (BioLegend, 100706); APC anti-mouse CD8a Antibody (BD Pharmingen, 344 553035); PE/Cyanine7 anti-mouse CD3 Antibody (BioLegend, 100220); APC anti-345 mouse IFN-γ Antibody (Invivogen, 17-7311-82); PE-Labeled Mouse H-346 2Kb&B2M&OVA (SIINFEKL) Tetramer Protein (Acrobiosystems, H2A-MP2H7); 347 Capicua Antibody (Novusbio, NB110-59906); anti-FLAG Antibody (Sigma-Aldrich, 348 F3165); β-actin (Sigma-Aldrich, A2228); Bim Antibody (Cell Signaling Technology, 349 2933T); Bcl-2 Antibody (Cell Signaling Technology, 3498T); Bax Antibody (Cell 350 Signaling Technology, 2772T); Bcl2 antibody (Proteintech, 68103-1-Ig); BAX 351 antibody (Proteintech, 60267-1-Ig); KEAP1 antibody (Proteintech, 10503-2-AP); 352 Anti-mouse Ig HRP (Rockland Immunochemicals, 18-8817-33); Anti-rabbit IgG HRP 353 (Rockland Immunochemicals, 18-8816-33); HRP-conjugated Mouse anti-Rabbit IgG 354 Light Chain (ABclonal, AS061); Prestained Protein Ladder 10-180 kDa (Thermo, 355 26616); Prestained Protein Ladder 25-400 kDa (Sevenbio, SW179). .. 356 4.2 Cells 357 CTLL-2 cells were purchased from the Cell Resource Center, Peking Union Medical 358 College (IBMS, CAMS/PUMC) and cultured in RPMI-1640 (Gibco) supplemented 359 with 10% fetal bovine serum (FBS) and 1× penicillin–streptomycin (Invitrogen), 10 360 mM HEPES, 1 mM Sodium pyruvate (Gibco), 2mM GlutaMAX (Gibco), and 100 361 U/ml IL-2.

    Incubation:

    Article Title: Epigenetic activation of PDLIM7 via H3K27 acetylation mitigates neuroinflammation and neurodegeneration in parkinson’s disease models
    Article Snippet: Parkinson’s disease (PD) is a chronic neurological disorder predominantly affecting middle-aged and older populations, with a prevalence second only to Alzheimer’s disease, impacting about 1% of individuals over the age of 65 worldwide (Tolosa et al. 2021; Lesage and Trinh 2023).. After cardiovascular and cerebrovascular illnesses and tumors, PD is currently the “third killer” of middle-aged and older persons in China.. Based on reliable statistics, it is predicted that there would be up to 4.94 million PD sufferers in China by 2030 (Li et al. 2019).

    other:

    Article Title: Biomimetic hybrid nanocarriers hitchhike neutrophils for targeted ginsenoside Rc delivery to BMSCs: ameliorating postmenopausal osteoporosis via iron homeostasis regulation.
    Article Snippet: Primary antibodies targeting p53(# 2524), Cleaved-Caspase 3(#9661S), Bax(#2772S) and Bcl-2(# 3498S) were acquired from Cell Signaling Technology (Beverly, USA).

    Control:

    Article Title: A novel PGK1 activator improves Alzheimer's disease by regulating glycolysis.
    Article Snippet: .. Primary antibodies used were as follows: PGK1 (1:1000, Abcam, ab199438), GAPDH (1:1000, Zsbio, TA-08), β-Tubulin (1:1000, Zsbio, TA10), Bcl-2 (1:1000, Cell Signaling Technology, 3498), Bax (1:1000, Cell Signaling Technology, 2772), APP (1:1000, Cell Signaling Technology, 2452), PS1 (1:1000, Cell Signaling Technology, 5643), and Aβ1-42 (1:1000, Abcam, ab180956). β-Tubulin or GAPDH served as the internal loading control. ..



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